Anti-SH3PX1/SNX9 Antibody Picoband™

Product Name

Anti-SH3PX1/SNX9 Antibody Picoband™

View all SH3PX1 Antibodies

SKU/Catalog Number

A03796-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SH3PX1/SNX9 Antibody Picoband™ catalog # A03796-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SH3PX1/SNX9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03796-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SH3PX1/SNX9 recombinant protein (Position: F211-Q573).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03796-2 is reactive to SNX9 in Human

Applications

A03796-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

78 kDa

Calculated molecular weight

66.592kDa

Background of SH3PX1

Sorting nexin-9 is a protein that in humans is encoded by the SNX9 gene. This gene encodes a member of the sorting nexin family. Members of this family contain a phosphoinositide binding domain, and are involved in intracellular trafficking. The encoded protein does not contain a coiled coil region, like some family members, but does contain a SRC homology domain near its N-terminus. The encoded protein is reported to have a variety of interaction partners, including of adaptor protein 2 , dynamin, tyrosine kinase non-receptor 2, Wiskott-Aldrich syndrome-like, and ARP3 actin-related protein 3. The encoded protein is implicated in several stages of intracellular trafficking, including endocytosis, macropinocytosis, and F-actin nucleation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SH3PX1/SNX9 using anti-SH3PX1/SNX9 antibody (A03796-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3PX1/SNX9 antigen affinity purified polyclonal antibody (Catalog # A03796-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3PX1/SNX9 at approximately 78 kDa. The expected band size for SH3PX1/SNX9 is at 67 kDa.

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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-SH3PX1/SNX9 antibody (A03796-2).
Overlay histogram showing MCF-7 cells stained with A03796-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH3PX1/SNX9 Antibody (A03796-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SNX9 (Source: Uniprot.org, NCBI)

Alternative Names

MST155; MSTP155; Protein SDP1; SDP1; SH3 and PX domain-containing protein 1; SH3PX1WISP; SH3PXD3ASH3 and PX domain-containing protein SH3PX1; sorting nexin 9; sorting nexin-9; WiskSH3 and PX domain-containing protein 3A SNX9 SDP1, SH3PX1, SH3PXD3A, WISP sorting nexin 9 sorting nexin-9|SH3 and PX domain-containing protein 1|SH3 and PX domain-containing protein 3A|Wiskott-Aldrich syndrome protein (WASP) interactor protein

For more info on SNX9, check out the SNX9 Infographic

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In this infographic, you will see the following information for SNX9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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